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bacterial expression and kinetic analysis of carboxylesterase

Bacterial Expression and Kinetic Analysis of Carboxylesterase

The thermocycler program was 98 °C for 30 s, followed by 30 cycles of 98 °C for 12 s, 60 °C for 25 s, 72 °C for 2 min, and with a final extension of 72 °C for 10 min. The PCR products were gel-purified, ligated into the pGEM-T easy vector after an adenine was added at the 3′ terminus, and used to transform JM109 cells.

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induction of a peptide with activity against a broad spectrum

Induction of a Peptide with Activity against a Broad Spectrum

After hybridization of the sequencing primer to the single-stranded products, 18 cycles of base incorporation were carried out on the 1G analyzer according to the manufacturer's instructions. Image analysis and base calling were performed using the Illumina Pipeline and sequence tags were obtained after purity filtering.

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ijms | free full-text | bacterial expression and kinetic

IJMS | Free Full-Text | Bacterial Expression and Kinetic

Carboxylesterasesare an important class of detoxification enzymes involved in insecticide resistance in insects. A subgroup of Helicoverpa armigera esterases, known as Clade 001, was implicated in organophosphate and pyrethroid insecticide resistance due to their overabundance in resistant strains. In this work, a novel carboxylesterasegene 001D of H. armigera from China was cloned, which has

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practical cell-free protein synthesis system using purified

Practical cell-free protein synthesis system using purified

Purity of isolated si/miRNAs (i.e., the fact that their sizes were 21-25nt) was verified through electrophoresis in a 15% polyacrylamide gel (GE Healthcare Amersham, UK), which was stained with

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pfmsa180 is a novel plasmodium falciparum vaccine antigen

PfMSA180 is a novel Plasmodium falciparum vaccine antigen

The purified proteins were resolved on a 12.5% SDS-polyacrylamide gel and stained with Coomassie show any high peaks suggesting the 32 to 36 hpi) were purified (>95% purity) by magnetic

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dual plug-and-display synthetic assembly using orthogonal

Dual Plug-and-Display Synthetic Assembly Using Orthogonal

Engineering modular platforms to control biomolecular architecture can advance both the understanding and the manipulation of biological systems. Icosahedral particles uniformly displaying single antigens stimulate potent immune activation and have been successful in various licensed vaccines. However, it remains challenging to display multiple antigens on a single particle and to induce

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massimo zacchini and paras ranjan pujari technological eco

Massimo Zacchini and Paras Ranjan Pujari Technological Eco

produce biomass and other bio-products for multiple profitable uses, avoiding the metal transfer to the food chain [9]. Because of its biological characteristics, such as rapid growth, high biomass production, wide root system, high genetic variability, remarkable ability to adapt to different environmental conditions, and

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akirins in sea lice: first steps towards a deeper

Akirins in sea lice: First steps towards a deeper

The purity of recombinant proteins was assayed by densitometry scanning of protein gels. Protein purification from adults was performed using Tri-reagent (Promega) according to the manufacturer′s instructions. 2.9. Protein gel electrophoresis and western blotting

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rubber high explosive: topics by science.gov

rubber high explosive: Topics by Science.gov

High frequency testing of rubber mounts.. PubMed. Vahdati, Nader; Saunders, L Ken Lauderbaugh. 2002-04-01. Rubber and fluid-filled rubber engine mounts are commonly used in automotive and aerospace applications to provide reduced cabin noise and vibration, and/or motion accommodations.

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olfactory proteins mediating chemical communication in the

Olfactory Proteins Mediating Chemical Communication in the

Typically, sex pheromones and other attractants (aka semiochemicals) are identified by a bioassay-guided isolation of natural products. Alternatively, olfactory proteins may be used in a reverse chemical ecology approach [12] , [13] for screening potential attractants on the basis of their affinity to odorant-binding proteins.

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induction of a peptide with activity against a broad spectrum

Induction of a Peptide with Activity against a Broad Spectrum

After hybridization of the sequencing primer to the single-stranded products, 18 cycles of base incorporation were carried out on the 1G analyzer according to the manufacturer's instructions. Image analysis and base calling were performed using the Illumina Pipeline and sequence tags were obtained after purity filtering.

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mitofusins, fzo homologs and functional derivatives thereof

Mitofusins, Fzo Homologs and functional derivatives thereof

Aliquots from each column fraction were electrophoresed on an SDS-polyacrylamide gel and stained with Coomassie Blue. Fractions with the highest degree of purity and highest concentration of the fusion protein were dialyzed in 1.5 M urea, 0.1 M Na phosphate, 0.01 M Tris-HCl, and 500 mM NaCl, pH 7. Generation of Antibodies to Fzo Amino Acid

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